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Dilution Equation

Dilution Equation; C1V1 = C2V2; M1V1 = M2V2
  • C1 is the concentration of the stock solution.
  • V1 is the volume to be removed (i.e., aliquoted) from the concentrated stock solution.
  • C2 is the final concentration of the diluted solution.
  • V2 is the final volume of the diluted solution. This is the volume that results after V1 from the stock solution has been diluted with diluent to achieve a total diluted volume of V2.
  • An alternative and commonly-used notation for this equation is M1V1 = M2V2, where M is used in place of C. Therefore, C1V1 = C2V2 and M1V1 = M2V2 are equivalent and simply different notations for the same equation.

Dilution Calculator - Molarity, Percent

Each calculator cell shown below corresponds to a term in the formula presented above. Enter appropriate values in all cells except the one you wish to calculate. Therefore, at least three cells must have values, and no more than one cell may be blank. The value of the blank cell will be calculated based on the other values entered. After a calculation is performed, the calculated cell will be highlighted and subsequent calculations will calculate the value of the highlighted cell (with no requirement to have a blank cell). However, a blank cell has priority over a highlighted cell.

For convenience, this calculator allows you to select different volume and concentration units, and the necessary conversions are carried out for you to obtain the value of the blank cell in the desired unit.

Stock concentration
mM
Volume from stock
mL
Final concentration
mM
Final solution volume
mL
  

Comments and/or Preparation Instructions

Relevant comments and/or instructions will appear here after a calculation is performed.




Posted: Thursday, March 1, 2001
Last updated: Saturday, March 22, 2025
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